The rapid growth of the global population provides significant challenges in actuality due to an increased demand for water. The constant pressure on water resources has caused unsustainable and inefficient management of the natural supplies. Recently, the increase of chemical products used in food production has contributed significantly to environmental degradation, threatening the availability of drinking water. This study is an analysis of the pH, electrical conductivity (EC), turbidity, total dissolved solids (TDS), temperature, dissolved oxygen (DO), salinity, and resistivity of water in 6 locations of the Lake Basin. The measured values were compared with Mexican and international standards to determine the degree of contamination at the sampling sites. The majority of measured data in test sites are in an acceptable range, with the exception of Yunuen Island, which shows an electrical conductivity and salinity that exceeds the values of the norms as well as dissolved solids that are close to the limit. Although the islands of Janitzio and Yunuen are located inside of Lake Pátzcuaro, only the island of Yunuen presents a probable pollution that can be associated with anthropogenic activities.